Effect of herbal extracts on bone regeneration in a rat calvaria defect model and screening system
이동환, 김일규, 조현영, 서지훈, Jang Jun-Min, 김진,
소속 상세정보
이동환 ( Lee Dong-Hwan ) - Inha University School of Medicine Department of Oral and Maxillofacial Surgery
김일규 ( Kim Il-Kyu ) - Inha University School of Medicine Department of Oral and Maxillofacial Surgery
조현영 ( Cho Hyun-Young ) - Inha University School of Medicine Department of Oral and Maxillofacial Surgery
서지훈 ( Seo Ji-Hoon ) - Inha University School of Medicine Department of Oral and Maxillofacial Surgery
( Jang Jun-Min ) - Inha University School of Medicine Department of Oral and Maxillofacial Surgery
김진 ( Kim Jin ) - Yonsei University College of Dentistry Department of Oral Pathology
Abstract
Objectives: The aim of this study was to evaluate the effects of herbal extracts on bone regeneration. Two known samples were screened.
Materials and Methods: We previously established a rat calvaria defect model using a combination of collagen scaffold and herbal extracts. An 8 mm diameter trephine bur with a low-speed dental hand piece was used to create a circular calvaria defect. The experimental group was divided into 4 classifications: control, collagen matrix, Danshen with collagen, and Ge Gan with collagen. Animals in each group were sacrificed at 4, 6, 8, and 10 weeks after surgery, and bone regeneration ability was evaluated by histological examination.
Results: Results revealed that both Danshen and Ge Gan extracts increased bone formation activity when used with collagen matrix. All groups showed almost the same histological findings until 6 weeks. However, after 6 weeks, bone formation activity proceeded differently in each group. In the experimental groups, new bone formation activity was found continuously up to 10 weeks. In the Danshen and Ge Gan groups, grafted materials were still present until 10 weeks after treatment, as evidenced by foreign body reactions showing multinucleated giant cells in chronic inflammatory vascular connective tissue.
Conclusion: Histological analyses showed that Danshen and Ge Gan extractions increased bone formation activity when used in conjunction with collagen matrix.
키워드
Bone regeneration; Herb extract; Danshen; Ge Gan; Rat calvaria defect
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